Evaluation of susceptibility and response in the surface of agents of surface mycoses (Trichophyton mentagrophytes; T. tonsurans) to antifungal drugs of interest in a medical clinic

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Claudio José dos Santos Júnior
http://orcid.org/0000-0002-2853-1968
Andressa Rafaelle de Lima Melo
http://orcid.org/0000-0002-9652-6239
Jéssica Maria Diniz do Nascimento
http://orcid.org/0000-0002-8132-7448
Silvana Maria Teixeira da Silva
http://orcid.org/0000-0001-7614-6610
Maria Anilda dos Santos Araújo
Aryanna Kelly Pinheiro Souza
http://orcid.org/0000-0003-1235-9144

Abstract

Introduction: The resistance of fungal species to drugs usually used in clinics is of great interest in the medical field. Objective: To evaluate susceptibility and in vitro response of species of Trichophyton spp. to antifungal drugs of interest in clinical medicine. Methods: 12 samples of clinical isolates from humans were used, nine of T. mentagrophytes and three of T. tonsurans. Susceptibility tests were performed according to the agar diffusion (AD) and broth microdilution (BM) methods. Results: In the AD method, the species T. tonsurans presented a percentage of sensitivity of 33% in relation to amphotericin B and 66% to itraconazole, with 100% resistance to ketoconazole and fluconazole. T. mentagrophytes also showed 100% resistance to ketoconazole in this technique, with 11% sensitivity to ketoconazole, 22% to itraconazole and 22% of samples classified as sensitive dose dependent. In the MC method, the species T. tonsurans presented a sensitivity percentage of 66%, 55% and 33% in relation to ketoconazole, fluconazole and itraconazole, respectively. The T. mentagrophytes species presented sensitivity percentages of 11%, 11%, 33% and 55% for amphotericin B, itraconazole, ketoconazole and fluconazole, respectively. Conclusion: There was resistance in vitro of the species of T. mentagrophytes and T. tonsurans against the antifungal fluconazole and relative resistance against ketoconazole in the AD method. In BM, however, important percentages of sensitivity were observed for the two species analyzed in relation to the antifungals fluconazole and ketoconazole when compared to itraconazole and amphotericin B.

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Santos Júnior, C. J. dos, Melo, A. R. de L., Nascimento, J. M. D. do, Silva, S. M. T. da, Araújo, M. A. dos S., & Souza, A. K. P. (2021). Evaluation of susceptibility and response in the surface of agents of surface mycoses (Trichophyton mentagrophytes; T. tonsurans) to antifungal drugs of interest in a medical clinic. ABCS Health Sciences, 46, e021203. https://doi.org/10.7322/abcshs.2019162.1431
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Original Articles

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