Viability of the random pattern dorsal skin flap in mice submitted to photobiomodulation and therapeutic ultrasound

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Laís Coan Fonanela
Jaquelini Betta Canever
Aderbal Silva Aguiar Júnior
Rafael Inácio Barbosa
Marisa de Cássia Registro Fonseca
Heloyse Uliam Kuriki
Lais Mara Siqueira das Neves
Alexandre Marcio Marcolino


Introduction: The skin flap is a surgical technique widely used in clinical practice and generally presents postoperative complications. Therefore, elucidating interventions that assist in tissue conservation is essential. Photobiomodulation (PBM) and therapeutic ultrasound (TUS) are non-invasive alternatives for assisting tissue repair, however, there is no consensus on the parameters used. Objective: To describe the effectiveness of the different parameters of PBM and TUS in the viability of the dorsal random pattern skin flap in mice. Methods: Fifty-five Swiss mice were used, distributed in eleven groups. The animals were submitted to surgical technique including revascularization of the area limited through a plastic barrier (polyester/polyethylene) with the same dimension as the flap. PBM or TUS was applied for five consecutive days. Photographic and thermographic recordings were performed with Cyber-Shot DSC-P72 and FlirC2 cameras and analyzed using the ImageJ® and FLIR Tools software, respectively. In the statistical analysis, the data were submitted to the GraphPad Prism® 8.0 software. Analysis of variance (ANOVA Two-way) and Tukey's post-test was performed, considering 5% significance level. Results: Groups 5 (PBM 830 nm; 10 J/cm²) and 6 (TUS 3 MHz; 0.4 W/cm²) showed percentages of viable tissue significantly higher on the third and fifth day of the experiment, when compared to the other groups. The temperature decreased significantly in group 1 when compared to the others in the postoperative period. Conclusion: The continuous TUS at 3 MHz and PBM 830 nm were more effective in improving the viability of the dorsal random pattern skin flap in mice.


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Fonanela, L. C., Canever, J. B., Aguiar Júnior, A. S., Barbosa, R. I., Fonseca, M. de C. R., Kuriki, H. U., Neves, L. M. S. das, & Marcolino, A. M. (2022). Viability of the random pattern dorsal skin flap in mice submitted to photobiomodulation and therapeutic ultrasound. ABCS Health Sciences, 47, e022227.
Original Articles


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